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Interlab G26 Users Manual Ver. Users Manual Ver. For any doubt please contact the Technical Service Interlab. If so Interlab disclaims any liability for damages, performance does not meet the features stated or erroneous results.

In case of variation, Interlab disclaims any liability for damages, performance does not meet the specifications stated or erroneous results. The device was designed and constructed to ensure, during normal use, an adequate level personal and collective security, in accordance with existing rules.

Be careful, however, the conduct rules here below. Before connecting the unit to the electricity grid, check that electrical system is fitted with an efficient ground. To ensure greater safety in case opening protective cover during the operation, all moving parts will stop after completing the movement and during migration electrophoresis the applied voltage is canceled. You understand and implement all precautions required in compliance with the regulation biological risk, in addition to what follows.

Wear all personal protective equipment required. Risk of electric shock. Risk of burns. Risk of biological contamination. Warning for the Service Personnel: unplug the power supply before working on the machine. The INTERLAB G26 is designed to be used with procedures established by Interlab and components manufactured and supplied by Interlab kit containing agarose gel plates, shelves, sponges soaked in buffer solution, reagent solution for staining, washing and destaining of the gel.

The optimal functioning instrument and the reliability of the results are guaranteed only when used components supplied by Interlab and do not observe the safety rules. The incorrect application of the recommended procedure may affect the results. Interlab can not be held liable for damages to persons or property, validity and reliability of the results obtained, the characteristics reported for the instrument, if: We do not observe the safety rules.

The operating environment where the equipment is installed does not comply with the requirements in this manual. The electrical system in the room where the equipment is installed is not lawful in the country and its safety guidelines.

The instrument is not used in accordance with the instructions in this manual. The manufacturer, installer or importer can not be held responsible for any loss data stored or disclosure sensitive personal data. Windows is a registered trademark of Microsoft Corporation. Programs Elfolab, Interlab G26 and accompanying documentation are protected by the laws on copyright.

The manufacturer declares the product complies with EC directives on medical devices in vitro diagnostic IVD. Its marking plate showing the characteristic data instrument, as the manufacturer and serial number is located on the lower right side instrument.

Purpose The purpose of the manual is to allow operators instrument to take those measures and provide all necessary human and material resources for its proper use and safe. Contents This manual contains all the information necessary for the installation, operation and maintenance of the instrument.

The scrupulous observance of what it is described guarantees a high degree safety and productivity of the instrument. Keeping and consulting the manual This manual is part of the instrument. It must be kept carefully and must be always available for consultation, either by user both the installation and maintenance workers.

In this manual the points of great importance are highlighted symbols below. Traders are advised to consult the important installation instructions and operating instructions contained in this document and especially to observe directions, regulations and prohibitions identified the following symbols:. With this symbol is expressed significant memorandum practice for the prevention of injuries or property damage. The failure to follow marked with this symbol during the operation, maintenance, repair or handling of the instrument can cause serious injury or even death.

With this symbol is expressed a note of particular importance. The failure to follow marked with this symbol during the operation, maintenance, repair or handling of the instrument can cause minor injuries, equipment damage, or loss important data.

With this symbol is expressed as a note indicating the optimal use of the facility are important reminders. The INTERLAB G26 is a diagnostic medical device in vitro for electrophoretic analysis of serum proteins, of unconcentrated urine proteins, of concentrated urine proteins, of hemoglobin, of lipoproteins, of isoenzymes and for the execution of several methods of immunofixation and isoelectric focusing. The G26 INTERLAB by the separation of different protein fractions using the principle of electrophoresis, that the difference in mobility between electrically charged molecules that orient and migrate with different rates when subjected to an electric field.

The migration is performed at constant temperature, obtained through the use of Peltier effect modules on agarose gel plates containing a specific buffer solution according to the method to execute. The gel acts as a support and molecular sieve and allows the different fractions could be resolved on the basis of net charge possessed. After electrophoresis, the gel is dried at a prescribed temperature, developed by appropriate staining, destained and dried. For methods that also allow the quantitative assessment, the measurement method is based on optical densitometry reading of a source diode LED and a photodiode detector.

The signal obtained is sent to the PC to be processed. The management of all phases of the analytical procedure is done through a software system firmware factory installed on the instrument and a software user interface installed on a PC that also returns the results of the analysis and allows the processing and storage. The INTERLAB G26 is a new-generation device that allows, for the majority of analytical methods, to run in full automation, the various stages of clinical electrophoresis on agarose gel, minimizing operator intervention.

INTERLAB G26 without the sampler that runs the following steps: dispensation of samples on agarose gel; electrophoretic migration; drying the gel; gel staining; gel destaining; gel drying; densitometric reading of the gel; acquisition and data processing.

INTERLAB G26 with the sampler that runs the following steps: identification of the tubes by reading a bar code; sampling of samples from tubes; deposit of samples in the sample plate; dispensation of samples on agarose gel; electrophoretic migration; drying the gel; gel staining; gel destaining; gel drying; densitometric reading of the gel; acquisition and data processing.

The system has great flexibility, allows a rational and efficient management of time for analysis, with significant increase in speed of analysis and the ability of the machine, thanks to: - High degree automation; - The wide variety of programs you can use; - The various options you can use; - The ability to schedule work sessions for the development of methods in order of priority that can be changed if necessary; - The possibility of processing methods in parallel, taking advantage of downtime from one phase to the next.

With the INTERLAB G26, you can execute with complete automation, the following analysis: Protein electrophoresis of serum and of concentrated urines; protein electrophoresis of serum and of concentrated urines; Protein expanded electrophoresis of serum and of concentrated urines; protein expanded electrophoresis of serum and of concentrated urines; Electrophoresis of the proteins in High Resolution; Electrophoresis of the concentrated proteins in High Resolution;.

Electrophoresis of the alkaline hemoglobins; Electrophoresis of the acid hemoglobins; Electrophoresis of the lipoproteins. You can execute, with semiautomatic procedure, the following analysis: Immunofixation of serum and of concentrated urines in Acid Blue; Immunofixation of serum and of concentrated urines in Acid Violet; Bence-Jones immunofixation of unconcentrated urines; Bence-Jones Immunofixation of concentrated urines; Pentavalent immunofixation of serum and of concentrated urines; Electrophoresis of the lactate dehydrogenase isoenzymes LDH ; Electrophoresis of the creatine kinase isoenzymes CK ; Electrophoresis of the alkaline phosphatase isoenzymes ALP ; Cryoglobulins; CSF isoelectric focusing; SDS proteinurie; Electrophoresis of the -Amylase isoenzymes; 1-Antitrypsin isoelectric focusing;.

NOTE All methods perform with the instrument are described individually in detail the procedures manual. Througt modules Temperature control during the migration Peltier effect. Type of tube used Exterior diameter mm only instrument with sampler Height mm. Reading bar codes While entering the rack only instrument with sampler. Time required for reading the 13 codes Min 5 sec. This product is indicated by selective collection for electrical disposal and electronic equipment under the WEEE Waste Electrical and Electronic Equipment.

Electronic products are not subject to selective harvesting are potentially hazardous to the environment and human health due the presence of harmful substances. Mechanical arm Driven by system software, couplet by means an eletromagnet and moves during the various stages of the analytical procedure: the needle dispenser, applicators, the holder of the agarose gel, the lids of the tanks of the dyes. Needle dispenser Take samples from tubes housed in racks and rack dispenses them in individual wells of the plate sample holder.

Site diluent container Here it is placed the 2 ml container of the diluent for immunofixation. Place of the needle wash After each sampling, the needle is washed repeatedly with distilled water so that you do not have a contamination between samples.

Wash applicator sites After each application of the gel, the applicators are repeatedly washed with the distilled water and make sure that there is no contamination between the previous and the following samples. Parking stain solution tank cover 1 blue It is the place where the cover of the tank is used during the staining of the stain solution 1.

Place of the decolouring treatment In this site the gel holder undergo the phase of decolouring. It is also used to wash the gel for immunofixation methods. Parking stain solution tank cover 2 violet It is the place where the cover of the tank is used during the staining of the stain solution 2.

Oven Here we begin the process of drying the gel at a temperature between 70 and 80 C using hot air. Parking dye tank cover 3 black It is the place where the cover of the tank is used during the staining of the stain solution 3. Optical drive Through a diode LED and a photodiode, the readings at the end gel densitometry of the whole analytical procedure.

The relevant data collected will be transferred to a PC for processing. Parking gel It is the place of the two frames where gel holder is taken at the beginning of the analysis and deposited at the end.

Site of the three stain solution tanks this is the place where the tanks 1, 2 and 3 are located. Site of the migration chamber Through the electrical contacts there is the flow of current during the electrophoretic migration. Plate migration The plate agarose gel adheres on it by creating the vacuum and the following three phases: dispensing of samples on the gel through the applicators; electrophoretic migration through an electric field; drying of the gel.

Use the Peltier effect to keep temperature constant during any phase of electrophoretic migration and drying. Site of the sample holder support Before the analysis you insert the sample plate support.

Site of the racks and rack rails This is the site of racks containing the tubes to allow the reading of the ID and sampling. Plate sample windows After each sampling carried out from the needle dispenser the window is closed to prevent the evaporation of the samples. Applicators Through the three applications 13 samples are taken from the plate sample one by one and are applied on the gel.

After each application, the applicator is decontaminated by washing in the place. Gel holder 1 and 2 They are a support of the gel plates during all the stages of the analysis. They allow us to work in parallel, the instrument can process two gel plates simultaneously. Tanks stain dyes 1, 2, 3, and their covers They contain three distinct colors: blue, violet and black for the operation of coloring after drying the gel.

Migration chamber Here there are the slots with the platinum electrodes where to place the sponges soaked in electrolyte solution in order to conduct the current in the gel. After the sampling on the gel, the migration plate rotates degrees clockwise by closing the migration chamber where the vacuum will be created. The conductive gel plate will adhere to the sponges and the gel will be subjected to the potential difference that will cause the electric field for electrophoresis.

Plate sample support It is laid the place disposable sample with 39 holes. Racks 1, 2, 3 It consists of 3 rows of 13 holes each in which there are the tubes with the samples. Through a bar code printed on each tube, the system is able to read the patient information and the type of analysis to read and transmit data to your PC.

To ensure optimum operation of the instrument and the reliability of the results follow the instructions below. Install the instrument in a room that meets the requirements for laboratory safety. Follow the instructions below for unpacking the instrument. Inspect carefully the instrument and accessories in the box enclosed within the same instrument.

Remove the four L-shaped metal fasteners that hold the outer casing of cardboard to the wooden base. Use a screwdriver inserted between the stopper and the base metal and force it downwards until the single firm has dropped.


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